Purification of Frog Oil Using Animal Bone-Base Activated Carbon

-Animal bones were carbonized at a temperatures of 800-900C .The carbonized sample was reduced to particle size of 75μm and then chemically activated at 500C using orthophosphoric acid as activating agent. The mixture was separated by centrifugation. The activated carbon was then added (1:8 v/w) to the frog oil for purification. Physicochemical properties and individual fatty acid profiles were monitored hourly. Melting point, odour and colour were determined for crude and purified samples. The result showed that peroxide value (PV) decreased from 3.50-1.80, while pH decreased from 11.8-9.0 after purification. Refractive index melting point reduced from 1.4876-1.4616 and 27.4-24.5C respectively. With odour and colour improved after 4 hrs. The same trend was observed in the iodine values, oleic, linolic, stearic,and palmitic acids, for frog oil after purification. The Freundlich isotherm model for the purification of frog oil using animal bone based activated carbon showed n and k values of 1.10 and 4.745 respectively with an R of 99%. The present result has shown that animal bone based activated carbon is quite suitable for the purification of frog oil. Keywords-Animal bone, carbonized, oil, crude, orthophosphoric acid